Brianna Bibel on Instagram: "When you order a cDNA plasmid (the DNA copy of the mature messenger RNA (mRNA) instructions for making a protein) from a repository (e.g. addgene, DNASU), it will likely come in some sort of generic cloning plasmid. And you will want to “subclone” it into an expression plasmid if you want to use it to make protein from it (i.e. move it out of the one plasmid and into another). https://youtu.be/ws6C8X7reYs blog form: https://bit.ly/vector_plasmids ; longer video: http

Brianna Bibel on Instagram: "In molecular cloning, we take a gene* from one place and (most commonly) stick it into a small circular piece of DNA called a PLASMID VECTOR**. Now we have "recombinant DNA." A lot of the times when we’re cloning we’re doing “subcloning” where we’re moving a gene from one plasmid to another (like moving it from the generic plasmid it came in when we ordered it) and putting it into a plasmid that’s ideal for protein-making. https://youtube.com/shorts/s0Hf3jWmQ2Q *for